Embryo freezing is the process of preserving the viability of sperm, oocytes (egg cells), or embryos by freezing them at ultra-low temperatures using liquid nitrogen. This method is applied both when multiple embryos are obtained during IVF treatment and when women wish to preserve fertility for medical reasons.
Sperm Retrieval via Micro-TESE
Micro-TESE (Microscopic Testicular Sperm Extraction) is a surgical procedure performed under a microscope to retrieve sperm directly from the testicular tissue. It is particularly used in cases of azoospermia (no sperm in the ejaculate). With magnification, less testicular tissue is removed, and the chance of finding sperm increases. Research shows that Micro-TESE improves sperm retrieval rates by 18–25%.
Ovulation Induction (Fertility Medication)
Ovulation induction is used in women with ovulatory dysfunction. It involves stimulating the ovaries with medications to support egg development and induce ovulation. Hormone medications are administered orally or via injection, and follicle development is closely monitored by ultrasound.
Endometrial Co-Culture (Artificial Uterine Environment)
Endometrial co-culture is a method used especially in couples who have experienced repeated IVF failures. On day 21 of the menstrual cycle, a small biopsy of the uterine lining is taken to create an artificial uterine environment in the lab. Embryos are cultured in this environment before transfer. This approach may enhance embryo development and improve pregnancy chances.
Single Embryo Transfer Policy
In accordance with current regulations, single embryo transfer is becoming increasingly common to reduce the risk of multiple pregnancies. Under the regulation enforced in Turkey since March 6, 2010:
- Women under 35 may have only one embryo transferred in their first and second IVF attempts.
- In the third and subsequent attempts, up to two embryos may be transferred.
- Women aged 35 and over may have up to two embryos transferred, regardless of attempt number.
This policy significantly reduces the risk of multiple pregnancies. However, because it can limit overall success rates, the quality of the embryos selected becomes critically important. In our clinic, embryo selection is carried out with the highest laboratory standards, and implantation potential is thoroughly evaluated.
Increasing Pregnancy Chances Through Effective Embryo Freezing
When single embryo transfer is applied, vitrification of the remaining high-quality embryos is one of the most effective ways to preserve pregnancy potential. If pregnancy is not achieved with fresh transfer, frozen embryos can later be thawed and transferred.
The modern embryo freezing protocols used in our clinic allow patients to achieve maximum pregnancy rates from a single egg retrieval cycle. This approach helps reduce the risk of multiples while supporting each couple’s journey toward parenthood.
The Importance of the IVF Laboratory
It is essential to remember that one of the most critical factors determining IVF success is the technical quality and expertise of the IVF laboratory. Embryo culture, freezing, and transfer outcomes are directly linked to laboratory standards. At our clinic, all procedures are performed within a state-of-the-art laboratory infrastructure that meets international accreditation standards.
